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Purity was finally determined by HPLC-DAD, NMR and GC-MS analysis with a focus on the impurity THC. System 1 consisted of a normal phase silica column as well as cyclohexane and acetone–both spiked with the modifier pyridine–as mobile phases. After the chromatographic run the fractions containing THCA fractions were pooled, extracted with hydrochloric acid to eliminate pyridine and evaporated to dryness. Loading 1800 mg cannabis extract yielded 623 mg THCA with a purity of 99.8% and a THC concentration of 0.09%. System 2 was based on a reversed-phase C18 column combined with 0.55% formic acid and methanol as mobile phases. After pooling the THCA-containing fractions methanol was removed in a rotary evaporator.

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CBD For Depression, Does It Work? – Thera Green CBD

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How To Talk To Your Doctor About Cannabis

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Can CBD Have Negative Side Effects?

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CBD In South Carolina

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